Composite

Part:BBa_K4630301:Design

Designed by: Liu Hongrui   Group: iGEM23_WHU-China   (2023-10-10)


EL222,blue light activated inducible system with RFP reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 130
    Illegal AgeI site found at 355
    Illegal AgeI site found at 1503
    Illegal AgeI site found at 1615
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is inserted into pET-28a backbone and characterized in E. coli BL21(DE3). Bacteria are grown on solid LB medium with Kanamycin at 37 degrees for 10 hours and then blue light around 475nm is administered for 20 hours. For every 2 hours, bacteria are sampled to carry out measurement of mRFP1 expression.


Source

improvement of BBa_K2447501

References